FacebookTwitterGoogle+RedditEmail

Illegal GE Bacteria Detected in an Animal Feed Supplement

Genetically engineered (GE) bacteria have been found in riboflavin vitamin supplements intended for animal feed use according to newly published EU tests.

Contamination of food grade or animal feed supplements with GE bacteria is illegal in the European Union. In 2014, however, a German enforcement laboratory alerted EU officials to illegal GE bacterial contamination of a riboflavin supplement intended for animal feed. Further tests showed that the illegal contaminating strain was not among those the manufacturer claimed to be using.

The findings, just published in the journal Food Chemistry, were made by regulators from Germany and Italy who were sampling Chinese imports (Paracchini et al., 2017).

Riboflavin (vitamin B2) is an essential vitamin of vertebrate organisms. It is commonly used as a food additive for humans and animals. Until quite recently, all riboflavin supplements were chemically synthesised. However, riboflavin is now frequently produced by commercial fermentation using overproducing strains of GE bacteria.

According to EU biosafety regulations, no GMO bacterial strain, nor any DNA, is allowed to be present in commercial supplements. However, the contaminated sample of riboflavin contained viable strains of the genetically modified organism Bacillus subtilis. The researchers cultured and tested the contaminating bacterium and subsequent DNA sequencing showed it to be a production strain.

Further testing showed it to contain genomic DNA conferring resistance to the antibiotic chloramphenicol. In addition, the strain contained DNA extrachromosomal plasmids with other antibiotic resistance genes. These conferred resistance to the antibiotics, ampicillin, kanamycin, bleomycin, tetracycline, and erythromycin.

Correspondence between German diplomats, Chinese authorities, and the company, subsequently established that these antibiotic resistance genes constituted key differences between the strains the company claimed to be using and what was detected in Germany. Only the erythromycin and chloramphenicol resistance genes were acknowledged by the producer. Whether the altered strains had been used intentionally or were inadvertent contaminants is still not clear.

In 2015 a French testing laboratory found one riboflavin sample also contaminated with what is likely to be an identical bacterial strain, again from China (Barbau-Piednoir et al., 2015).

According to Janet Cotter of the consultancy Logos Environmental, European Food Safety Authority regulations state that antibiotic resistance genes “should be restricted to field trial purposes and should not be present in GM plants to be placed on the market” to prevent them entering the food chain as these antibiotic resistance genes presumably have. “This incident is the latest in a series of GMO escapes. It highlights the need for a database of detection methodologies for all GMOs used, both in contained use and at the field trial stage, so any escapees can at least be detected without facing the serious analytical challenges identification of this GMO contaminant posed.”

The publication documenting the tests also highlighted various altered properties of the detected Bacillus subtilis strain, including reduced fidelity of its protein translation system which might lead it to produce novel proteins.

References

Barbau-piednoir, E. Sigrid C. J. De Keersmaecker, Maud Delvoye, Céline Gau, Patrick Philipp and Nancy H. Roosens (2015) Use of next generation sequencing data to develop a qPCR method for specific detection of EU-unauthorized genetically modified Bacillus subtilis overproducing riboflavin. BMC Biotechnology 2015 15:103 DOI: 10.1186/s12896-015-0216-y

Paracchini, V., Petrillo, M., Reiting, R., Angers-Loustau, A., Wahler, D., Stolz, A., … & Pecoraro, S. (2017). Molecular characterization of an unauthorized genetically modified Bacillus subtilis production strain identified in a vitamin B 2 feed additive. Food Chemistry, 230, 681-689.

More articles by:

Jonathan Latham edits Independent Science News.

Weekend Edition
November 16, 2018
Friday - Sunday
Jonah Raskin
A California Jew in a Time of Anti-Semitism
Andrew Levine
Whither the Melting Pot?
Joshua Frank
Climate Change and Wildfires: The New Western Travesty
Nick Pemberton
The Revolution’s Here, Please Excuse Me While I Laugh
T.J. Coles
Israel Cannot Use Violent Self-Defense While Occupying Gaza
Rob Urie
Nuclear Weapons are a Nightmare Made in America
Paul Street
Barack von Obamenburg, Herr Donald, and Big Capitalist Hypocrisy: On How Fascism Happens
Jeffrey St. Clair
Roaming Charges: Fire is Sweeping Our Very Streets Today
Aidan O'Brien
Ireland’s New President, Other European Fools and the Abyss 
Pete Dolack
“Winners” in Amazon Sweepstakes Sure to be the Losers
Richard Eskow
Amazon, Go Home! Billions for Working People, But Not One Cent For Tribute
Ramzy Baroud
In Breach of Human Rights, Netanyahu Supports the Death Penalty against Palestinians
Brian Terrell
Ending the War in Yemen- Congressional Resolution is Not Enough!
John Laforge
Woolsey Fire Burns Toxic Santa Susana Reactor Site
Ralph Nader
The War Over Words: Republicans Easily Defeat the Democrats
M. G. Piety
Reading Plato in the Time of the Oligarchs
Rafael Correa
Ecuador’s Soft Coup and Political Persecution
Brian Cloughley
Aid Projects Can Work, But Not “Head-Smacking Stupid Ones”
David Swanson
A Tale of Two Marines
Robert Fantina
Democrats and the Mid-Term Elections
Joseph Flatley
The Fascist Creep: How Conspiracy Theories and an Unhinged President Created an Anti-Semitic Terrorist
Joseph Natoli
Twitter: Fast Track to the Id
William Hawes
Baselines for Activism: Brecht’s Stance, the New Science, and Planting Seeds
Bob Wing
Toward Racial Justice and a Third Reconstruction
Ron Jacobs
Hunter S. Thompson: Chronicling the Republic’s Fall
Oscar Gonzalez
Stan Lee and a Barrio Kid
Jack Rasmus
Election 2018 and the Unraveling of America
Sam Pizzigati
The Democrats Won Big, But Will They Go Bold?
Yves Engler
Canada and Saudi Arabia: Friends or Enemies?
Cesar Chelala
Can El Paso be a Model for Healing?
Mike Ferner
The Tragically Misnamed Paris Peace Conference
Barry Lando
Trump’s Enablers: Appalling Parallels
Ariel Dorfman
The Boy Who Taught Me About War and Peace
Yves Engler
Ottawa, Yemen and Guardian
Binoy Kampmark
The Disgruntled Former Prime Minister
Tracey L. Rogers
Dear White Women, There May be Hope for You After All
Faisal Khan
Is Dubai Really a Destination of Choice?
Arnold August
The Importance of Néstor García Iturbe, Cuban Intellectual
James Munson
An Indecisive War To End All Wars, I Mean the Midterm Elections
Nyla Ali Khan
Women as Repositories of Communal Values and Cultural Traditions
Dan Bacher
Judge Orders Moratorium on Offshore Fracking in Federal Waters off California
Christopher Brauchli
When Depravity Wins
Robby Sherwin
Here’s an Idea
Susan Block
Cucks, Cuckolding and Campaign Management
Louis Proyect
The Mafia and the Class Struggle (Part Two)
FacebookTwitterGoogle+RedditEmail